1. Summary of Technical Progress

1. Current Technical Status

2. Tasks of the work plan

• State / Situation at the beginning of the current quarter

Дописать 

• Fulfilled work

Дописать 

• Results by the end of the current quarter

Дописать 

Biological samples were taken from birds belonging to 2 species (Table 1).  

Table 1 

The list of birds from which samples on influenza virus were taken in Quarter 7 (December 2010- February 2011)  

• Personnel Commitments

• State / Situation at the beginning of the current quarter

At the project beginning molecular genetic researches of birds have been conducted. Virological researches on cell culture of dog’s kidney was conducted, however, there was no virus isolation. There were samples with cytopathogenic effects.  

• Fulfilled work

  Total number of conducted researches is 343.  33 species of birds were exposed to molecular-genetic researches, presence of influenza A were detected in 3 bird species.    

• Results by the end of the current quarter

Table 2

Results of monthly molecular-genetic researches of birds

Positive samples in PCR reaction in a Real time mode with the threshold line before 30 cycles, thus indicating the presence of sufficient RNA RNase P gene indicating the specimen is of acceptable quality. At the same time primary infection and 2 passages on a cell culture have been conducted out of them in 3 cases (from materials from Night Heron and 2 Mallards ) cytopathogenic virus effect is detected. These isolates are typed in hemagglutination reaction and hemagglutination inhibition of reaction. Preliminary results of virological researches see below.  
 
 
 
 
 
 
 

Table 3

Results of virological researches

Total number of conducted researches is 307.  46 species of birds were exposed to molecular-genetic researches ( table 4). Research were conducted by real time  PCR on influenza A, subtypes Н1, Н3 and  Н5. During researches of 46 bird species in 2 samples (0,6%)  from 1 bird species  presence of influenza A virus is detected, however results of sybtyping on Н1, Н3. we find  on sample  Н5 were negative Positive samples on influenza A were frozen at -70 Celsius degree for further researches. It is necessary to conduct researches on subtypes H2, H4, H6, H15 and N1-N9 with foreign collaborators. For this purpose business trips to laboratories of foreign collaborators to exchange experiences is necessary. 

Results of molecular-genetic investigations of birds in Quarter 6 (September-December, 2010)

                                                                                                       Table 4

Out of 307 researches birds, 293 have been researched in Issyk-Kul oblast. In Issyk-Kul oblast positive samples of avian influenza were found.                                       

  Table 5

 During analysis of influenza A virus detection among waterfowls it was determined that more often in the reporting period the positive A influenza sample have been detected among Mallards (2 positive).

Detection of influenza virus among various species of waterfowls in Quarter 6 (September-December, 2010)

                                                                                                                                                 Table 6  

• Personnel Commitments

Пусть бухгалтер посчитает правильно, пропустила дни у Динары. 
 
 

• State / Situation at the beginning of the current quarter

Дописать

• Fulfilled work

Дописать

• Results by the end of the current quarter

Дописать

• Personnel Commitments

• State / Situation at the beginning of the current quarter

Subtyping of viruses is conducted. It is planned to discuss the results of researches and the possibility to conduct strain sequencing with foreign collaborators.

• Fulfilled work

Допишите  выполненную работу

• Results by the end of the current quarter

Допишите  результат к концу текущего квартала

• Personnel Commitments

• State / Situation at the beginning of the current quarter

The epidemiological monitoring was not carried out in the Republic

• Fulfilled work

• Results by the end of the current quarter

Epizootological (epidemiological) monitoring of  Batken oblast

Batken oblast was formed in 1999 and is located on the south-western part of the country. Its area is 16,9 thousand km². There are 3 administrative districts, 4 administrative towns, 4 urban villages and 29 villages in the oblast. The oblast is bordering on the south-west and north-west with Tajikistan, and on the north-east with the Republic of Uzbekistan. The oblast’s territory is located on the northern slopes and valleys of the Turkestan and Alay mountain systems in the southern part of the Ferghana Valley. In the oblast there is a well developed network of river systems: Soh, Isfana, Isfara-Sai, Shaimerden, Ak-Suu, Kara-Suu and others with their numerous tributaries which have glacial supply and is the main source of irrigation of lands. Tortkul reservoir was built in 1972 in this oblast. There are no large natural lakes in the oblast. In the valleys there are many rivers, small lakes (river Shaimerden Abshyr-Sai and others). Mountain lakes from November to March get frozen and located at a distance of 20-40km from the settlements in Batken oblast and in fact have no epizootic significance.

25 6418 heads of chickens, including 184,940 hens are being raised and they are concentrated in the farms and individual entrepreneurs - 85,952 heads and in private households of citizens 98,988 heads.

There are no large poultry farms and poultry factories in the oblast.

1.Batken district

Batken administrative district is situated in the heart of the Batken oblast. The district consists of 9 rural districts, 44 settlements. The district’s administrative center is Batken town.

The district's area is 6.1 km². The territory of  the district is situated in Batken valley stretching from north to south and by small valleys in the canyons Soh Isfara on the northern slopes of  Alay-Turkestan ranges. The climate is continental. Major river arteries of the region are the rivers Soh and Isfara, which are the main sources of irrigation of areas requiring irrigation. Wild waterfowl lapwings, wild ducks, storks, etc can be found on floodplains and wetlands.

There are 50,289 heads of different types of poultry, including 38 092 heads of laying hens in Batken district. Out of total number of poultry in the district, 28,848 heads of laying hens are being raised in   farms and in individual entrepreneurs  and 9244 heads in private households.

Tortkul reservoir built in 1972 in Batken district, which originates from the river Isfara and regulates the regime of long-term use of water for irrigation of areas requiring irrigation.

The length of the reservoir is 5 km, width 2.1 km, the deepest place is 26.8 m, the mirror surface 6.57 km², water volume is 90 million m³.

Waterfowls arrive in Tortkul reservoir in the spring.

The most closely located settlements to Tortkul reservoir in Batken district are settlements: Min-Oruk, Kyzyl-Jol and Bujum of Batken district.

Village Min-Oruk of Samarkandek rural district of Batken district is located 3,5-4 km from Tortkul reservoir. The village has 1500 heads of chickens, 350 turkeys and 170 heads of ducks and geese.

Kyzyl-Jol settlement of Batken town is located 10 km away from Tortkul reservoirs and they have more than 5600 heads of chickens and more than 800 heads of ducks, geese and turkeys.

Buzhum village of Kara-Bulak rural district of Batken oblast is located at a distance of 10-11 km from Tortkul reservoir.

In village Buzhum there are more than 4500 heads of chickens, 500 turkeys and more than 290 heads of ducks and geese.

In  case of importation of the virus of highly pathogenic avian influenza by waterfowls the first affected settlements will be Min-Oruk, Kyzyl-Jol and Buzhum of Batken district of Batken oblast.

2. Kadamjai district

Kadamjai administrative district is located in the northeast of Batken oblast and concentrated on the southern part of  Ferghana Valley and on the northern slopes of Alai  low-lying ridges.

District was established in 1938. The district's area is 6.1 km². There are 11 rural districts, 4 towns and 88 villages in the district. The administrative center of the district is Kadamjai urban village.

The area is rich with natural deposits of antimony, mercury, coal, etc. The climate is continental. Summer is dry, warm and moderate cold winters.

The main waterways in the district; rivers Isfaram-Sai Shaimerden, Ak-Suu, and others. Water resources of the district are mainly used for irrigation of agricultural lands. In the floodplains, there are small aquatic birds, wild ducks, storks, lapwings, etc.  

On the upper rivers of Shaimerden in Kok-Suu canyon there is Kurban-Kul lake with 0.13 km² area and located at a distance of 30-40 km from the settlements.

River Kadamjay district takes its start from  glaciers, springs, and precipitation. High water of rivers starts from May to August.

There are 103,694 heads of different species of poultry including 73 869 heads of hens and they are concentrated in  (peasant) farms and individual enterprises 12 434 heads and 61,435 heads in private households of population of Kadamdjay district.

The role of migratory waterfowls in Kadamjai district in spread of bird flu pathogen is negligible.

3.Leylek District

Leylek district is located in southwest of Batken oblast and bordering from east with Batken distrcit, the rest rural areas with the Republic of Tajikistan. The district's area is 4.7 km². District was formed in 1928. The district’s administrative center is Isfana town.

Leylek district consists of 9 rural centers, 1 town of district subordination - Isfana town and 46 rural settlements. The climate is continental, summer dry, hot, and winters are moderately cold.

The main rivers: river Ak-Suu (inflows Dacat-Suu, Sardala, Sumbula), Kara-Suu, Leilek (tributaries: Oromo, Jeti-Kopuro, Kojo-Bakyrgan). Water resources of the district are widely used for irrigation of crops.

There are no lakes and pools of water daily regulation regimes on the territory of Leilek district.

In springs there are arrivals of migratory waterfowl (wild ducks, herons) generally for a short time.

Various species of poultry, total 58,908 heads, including hens 41,038 heads and 36,124 heads  in  (peasant) farms and individual entrepreneurs, as well as 4914 heads in private households of the population are being raised in Leilek district. There are no large poultry farms and factories.

Isfana

Isfana is town of district subordination, and the administrative center of Leilek district. The town has 8,159 heads of poultry, including 550 heads of ducks and geese. Out of 6,012 heads of laying hens   3,890 are being raised in farms and individual entrepreneurs and 2,122 heads in private households of the population.

Batken town

Batken town is the administrative center Batkens district and Batken oblast.  11,995 poultry including 10,814 heads of laying hens are being raised in the town. 6616 laying hens are being raised in (peasant) farms and individual entrepreneurs and 4198 in private households of the population.

Kyzyl-Kiya town

Kyzyl-Kiya was formed in 1901 as an urban village. Kyzyl-Kiya as an industrial town focused on coal production.

 25,930 heads of poultry  including 17,425 heads of egg-laying hens are and they are being raised in this town, 1930 heads in  farms and individual entrepreneurs, and 15,495 heads in private households.

Sulukta town

Sulukta was formed in mid-1800, as a result of the development of brown coal. Industrial town. There are 5,603 of poultry in Sulukta, including 3,702 heads laying hens and they are being raised in private households of the population.

Conclusion:

 Due to the absence of large lakes, reservoirs and short-term arrivals of migratory waterfowl there is no a big risk of importation of  avian influenza pathogen on the territory on the territory of Batken oblast of the Kyrgyz Republic.

• Personnel Commitments

• Fulfilled work

• Preparation of application on procurement of reagents and equipment on Flow 2.

• Procurement of necessary consumables for conduction of researches.

• Work coordination of institutes-participant.

• Financial reports

• Translation

• Personnel Commitments

2. Summary of Personnel Commitments

Personnel Changes: None